Review

asic2 (Alomone Labs)

Alomone Labs is a verified supplier

Alomone Labs manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Alomone Labs asic2
Asic2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/asic2/product/Alomone Labs
Average 93 stars, based on 31 article reviews

asic2 - by Bioz Stars, 2026-03

93/100 stars

Images

Image Search Results

( A ) Protocol of mouse tube test assay. Four mice housed together for 2 weeks undergo a round-robin tournament to determine social ranking. ( B ) Typical ranking of a group of one Asic2 −/− mouse versus three WT cagemates. ( C ) Average number of winning times in trials 1 and 7. ( D ) Comparison of winning times between WT and Asic2 −/− mice shows that Asic2 −/− mice have higher winning times. **** P < 0.0001, one-way ANOVA. n = 28 cages. ( E ) Typical ranking of three Asic2 −/− mice versus one WT cagemate. ( F ) Average number of winning times in trials 1 and 7. ( G ) Three Asic2 −/− mice exhibit higher winning times than one WT cagemate. **** P < 0.0001; ns, nonsignificant, one-way ANOVA. n = 7 cages. ( H ) Comparison of winning times between groups of one Asic2 −/− mouse versus three WT cagemates and one WT mouse versus three Asic2 −/− cagemates, using data from (B) to (G). *** P = 0.0007, two-tailed unpaired Student’s t test. ( I ) Comparison of winning times between groups of one Asic1a −/− mouse versus three WT cagemates, and one WT mouse versus three Asic1a −/− cagemates. ns, P = 0.8477, two-tailed unpaired Student’s t test. n = 7 cages. ( J and K ) Schematic of mouse warm spot test and correlation with tube test rank. P = 0.0118, two-sided Fisher’s exact test. n = 16 cages. ( L and M ) Schematic of mouse urine marking test and correlation with tube test rank. P = 0.0118, two-sided Fisher’s exact test. n = 16 cages.

Journal: Science Advances

Article Title: Mice lacking acid-sensing ion channel 2 in the medial prefrontal cortex exhibit social dominance

doi: 10.1126/sciadv.adn7573

Figure Lengend Snippet: ( A ) Protocol of mouse tube test assay. Four mice housed together for 2 weeks undergo a round-robin tournament to determine social ranking. ( B ) Typical ranking of a group of one Asic2 −/− mouse versus three WT cagemates. ( C ) Average number of winning times in trials 1 and 7. ( D ) Comparison of winning times between WT and Asic2 −/− mice shows that Asic2 −/− mice have higher winning times. **** P < 0.0001, one-way ANOVA. n = 28 cages. ( E ) Typical ranking of three Asic2 −/− mice versus one WT cagemate. ( F ) Average number of winning times in trials 1 and 7. ( G ) Three Asic2 −/− mice exhibit higher winning times than one WT cagemate. **** P < 0.0001; ns, nonsignificant, one-way ANOVA. n = 7 cages. ( H ) Comparison of winning times between groups of one Asic2 −/− mouse versus three WT cagemates and one WT mouse versus three Asic2 −/− cagemates, using data from (B) to (G). *** P = 0.0007, two-tailed unpaired Student’s t test. ( I ) Comparison of winning times between groups of one Asic1a −/− mouse versus three WT cagemates, and one WT mouse versus three Asic1a −/− cagemates. ns, P = 0.8477, two-tailed unpaired Student’s t test. n = 7 cages. ( J and K ) Schematic of mouse warm spot test and correlation with tube test rank. P = 0.0118, two-sided Fisher’s exact test. n = 16 cages. ( L and M ) Schematic of mouse urine marking test and correlation with tube test rank. P = 0.0118, two-sided Fisher’s exact test. n = 16 cages.

Article Snippet: Primary antibodies used in this study are anti-ASIC1a antibody (Alomone Labs, no. ASC-014), anti-ASIC2 antibody (Alomone Labs, no. ASC-012), GluA1 polyclonal antibody (Thermo Fisher Scientific, no. PA5-99527), NMDAR2B polyclonal antibody (Innovative Research, no. 71-8600), PKA alpha antibody (Thermo Fisher Scientific, no. MA5-37857), and β-actin antibody (Cell Signaling Technology, no. 4967).

Techniques: Comparison, Two Tailed Test, Spot Test

( A ) Recordings of acid-induced currents in the mPFC slice. ( B to D ) pH-dependent ASIC currents in WT (B) ( n = 5 to 13 cells per pH dose, from four mice), Asic1a −/− (C) ( n = 5 to 21 cells per pH dose, from four mice) and Asic2 −/− (D) neurons ( n = 5 to 12 cells per pH dose, from 4 mice). ( E ) Normalized ASIC currents in WT (black) and Asic2 −/− (red) neurons. ( F ) Current density at pH 5.0 in WT and Asic2 −/− neurons. ns, P = 0.2828. ( G ) Desensitization decay times (τ), ** P = 0.0058, n = 16 cells per four mice. ( H ) Normalized ASIC current per unit time. **** P < 0.0001, n = 15 cells per four mice. ( I ) Representative Western blot data illustrating the expression of ASIC1a and ASIC2 in WT mice with different rankings in the tube test. ( J ) Average expression data for ASIC1a and ASIC2. ns, P = 0.1592, ** P < 0.007. ( K and L ) Correlation curves depicting expressions of ASIC1a [Pearson correlation coefficient ( r ) = −0.23, P = 0.18, ns] and ASIC2 ( r = −0.80, ** P = 0.005) in relation to social rankings, n = 9 mice in each ranking group. ( M ) Representative ASIC currents in neurons from winner (α) and loser (δ) mice. ( N and O ) Average current density and τ of ASIC currents. ns, P = 0.4211; * P = 0.0113, n = 21 cells per four mice. ( P ) pH-dependent ASIC currents in WT mice were analyzed to determine pH 50 values, obtained by best-fitting with the Hill equation. ( Q ) Average pH 50 between neurons in α and δ mice. **** P < 0.0001, n = 13 to 15 cells per four mice.

Journal: Science Advances

Article Title: Mice lacking acid-sensing ion channel 2 in the medial prefrontal cortex exhibit social dominance

doi: 10.1126/sciadv.adn7573

Figure Lengend Snippet: ( A ) Recordings of acid-induced currents in the mPFC slice. ( B to D ) pH-dependent ASIC currents in WT (B) ( n = 5 to 13 cells per pH dose, from four mice), Asic1a −/− (C) ( n = 5 to 21 cells per pH dose, from four mice) and Asic2 −/− (D) neurons ( n = 5 to 12 cells per pH dose, from 4 mice). ( E ) Normalized ASIC currents in WT (black) and Asic2 −/− (red) neurons. ( F ) Current density at pH 5.0 in WT and Asic2 −/− neurons. ns, P = 0.2828. ( G ) Desensitization decay times (τ), ** P = 0.0058, n = 16 cells per four mice. ( H ) Normalized ASIC current per unit time. **** P < 0.0001, n = 15 cells per four mice. ( I ) Representative Western blot data illustrating the expression of ASIC1a and ASIC2 in WT mice with different rankings in the tube test. ( J ) Average expression data for ASIC1a and ASIC2. ns, P = 0.1592, ** P < 0.007. ( K and L ) Correlation curves depicting expressions of ASIC1a [Pearson correlation coefficient ( r ) = −0.23, P = 0.18, ns] and ASIC2 ( r = −0.80, ** P = 0.005) in relation to social rankings, n = 9 mice in each ranking group. ( M ) Representative ASIC currents in neurons from winner (α) and loser (δ) mice. ( N and O ) Average current density and τ of ASIC currents. ns, P = 0.4211; * P = 0.0113, n = 21 cells per four mice. ( P ) pH-dependent ASIC currents in WT mice were analyzed to determine pH 50 values, obtained by best-fitting with the Hill equation. ( Q ) Average pH 50 between neurons in α and δ mice. **** P < 0.0001, n = 13 to 15 cells per four mice.

Techniques: Western Blot, Expressing

( A ) Schematic showing the injection of AAV 2 -CMV-mASIC2 into the mPFC in Asic2 −/− mice. ( B ) Left, a representative image of the AAV 2 -mASIC2 injection site and neurons expressing mASIC2; middle-right, representative recording of acid-induced ASIC-like currents in the GFP positive and negative neurons. ( C ) The representative comparison of acid-induced currents among WT, Asic2 −/− (non-GFP cells), and AAV-mASIC2 neurons (GFP positive cells). ( D and E ) Comparison of desensitization decay times (D) and density (E) of the acid-induced currents in the neurons in (C). WT versus Asic2 −/− , ** P = 0.0012, Asic2 −/− versus AAV-mASIC2, ** P = 0.0082; AAV-mASIC2 versus AAV-sham, * P = 0.0129, ns, P = 0.6763, one-way ANOVA. n = 12 to 15 cells from four mice. ( F ) Schematic showing the tube test protocol before and after AAV-mediated expression of ASIC2 in the mPFC of Asic2 −/− mice. ( G ) Representative result of ranking in the tube test following the restoration of ASIC2 in mPFC neurons of Asic2 −/− mice. ( H ) Average winning times in the tube test after restoring ASIC2 in mPFC neurons of Asic2 −/− mice. ( I ) Comparison of winning times between the group with restored ASIC2 in Asic2 −/− mice and one of its WT subordinates. ** P = 0.0092; ns, P = 0.3652, two-tailed unpaired Student’s t test. n = 9 cages.

Journal: Science Advances

Article Title: Mice lacking acid-sensing ion channel 2 in the medial prefrontal cortex exhibit social dominance

doi: 10.1126/sciadv.adn7573

Figure Lengend Snippet: ( A ) Schematic showing the injection of AAV 2 -CMV-mASIC2 into the mPFC in Asic2 −/− mice. ( B ) Left, a representative image of the AAV 2 -mASIC2 injection site and neurons expressing mASIC2; middle-right, representative recording of acid-induced ASIC-like currents in the GFP positive and negative neurons. ( C ) The representative comparison of acid-induced currents among WT, Asic2 −/− (non-GFP cells), and AAV-mASIC2 neurons (GFP positive cells). ( D and E ) Comparison of desensitization decay times (D) and density (E) of the acid-induced currents in the neurons in (C). WT versus Asic2 −/− , ** P = 0.0012, Asic2 −/− versus AAV-mASIC2, ** P = 0.0082; AAV-mASIC2 versus AAV-sham, * P = 0.0129, ns, P = 0.6763, one-way ANOVA. n = 12 to 15 cells from four mice. ( F ) Schematic showing the tube test protocol before and after AAV-mediated expression of ASIC2 in the mPFC of Asic2 −/− mice. ( G ) Representative result of ranking in the tube test following the restoration of ASIC2 in mPFC neurons of Asic2 −/− mice. ( H ) Average winning times in the tube test after restoring ASIC2 in mPFC neurons of Asic2 −/− mice. ( I ) Comparison of winning times between the group with restored ASIC2 in Asic2 −/− mice and one of its WT subordinates. ** P = 0.0092; ns, P = 0.3652, two-tailed unpaired Student’s t test. n = 9 cages.

Techniques: Injection, Expressing, Comparison, Two Tailed Test

( A ) Recording site schematic in the mPFC. ( B to D ) PPR recorded with 50- to 500-ms interstimulus intervals. Left: Representative PPR traces at 50 ms in WT and Asic2 −/− mice before and after social rankings. Right: Average PPR plotted against intervals. (C) * P = 0.0433; (D) ** P = 0.0088, 0.0044, * P = 0.0481, n = 10 cells per four mice. ( E ) Representative AMPAR-EPSCs (−80 mV) and NMDAR-EPSCs (+60 mV) in WT and Asic2 −/− neurons. ( F ) Average AMPAR/NMDAR ratios before and after social rankings. Current amplitudes measured 70 ms after onset. ns, P = 0.6117; * P = 0.0470; *** P = 0.0006. n = 22 to 29 cells per five mice. ( G and H ) mEPSCs in the groups of WT versus WT, and WT versus Asic2 −/− mice after social rankings. Upper: Representative mEPSC traces. Lower: Cumulative distributions of mEPSC amplitudes and interevent intervals. (G) * P = 0.0342; ns, P = 0.4768; (H) * P = 0.0109, 0.0065, n = 18 cells per four mice. ( I ) AMPAR current rectification in WT and Asic2 −/− neurons before social rankings. Left: Current-voltage relationships of AMPARs. Inset: Representative AMPAR-EPSC traces at −80 mV and +60 mV. NMDARs blocked with 100 μM d -APV. Right: AMPAR current rectification index (−80 mV/+60 mV). ns, P = 0.5913. n = 18 cells per four mice. ( J and K ) AMPAR current rectification of WT versus WT, and WT versus Asic2 −/− neurons after social rankings. ns, P = 0.4467; *** P = 0.0003, n = 22 cells per four mice. ( L to N ) Time course of 50 μM NASPM effect on EPSCs in mPFC neurons of WT versus WT (M) and Asic2 −/− versus WT (N) mice groups before (L) and after (M and N) social rankings. NMDARs blocked with 100 μM d -APV. Right: NASPM-sensitive EPSC comparison. ns, P = 0.9864, P = 0.1059; **** P < 0.0001, n = 10 cells per four mice. All comparisons by two-tailed unpaired Student’s t test.

Journal: Science Advances

Article Title: Mice lacking acid-sensing ion channel 2 in the medial prefrontal cortex exhibit social dominance

doi: 10.1126/sciadv.adn7573

Figure Lengend Snippet: ( A ) Recording site schematic in the mPFC. ( B to D ) PPR recorded with 50- to 500-ms interstimulus intervals. Left: Representative PPR traces at 50 ms in WT and Asic2 −/− mice before and after social rankings. Right: Average PPR plotted against intervals. (C) * P = 0.0433; (D) ** P = 0.0088, 0.0044, * P = 0.0481, n = 10 cells per four mice. ( E ) Representative AMPAR-EPSCs (−80 mV) and NMDAR-EPSCs (+60 mV) in WT and Asic2 −/− neurons. ( F ) Average AMPAR/NMDAR ratios before and after social rankings. Current amplitudes measured 70 ms after onset. ns, P = 0.6117; * P = 0.0470; *** P = 0.0006. n = 22 to 29 cells per five mice. ( G and H ) mEPSCs in the groups of WT versus WT, and WT versus Asic2 −/− mice after social rankings. Upper: Representative mEPSC traces. Lower: Cumulative distributions of mEPSC amplitudes and interevent intervals. (G) * P = 0.0342; ns, P = 0.4768; (H) * P = 0.0109, 0.0065, n = 18 cells per four mice. ( I ) AMPAR current rectification in WT and Asic2 −/− neurons before social rankings. Left: Current-voltage relationships of AMPARs. Inset: Representative AMPAR-EPSC traces at −80 mV and +60 mV. NMDARs blocked with 100 μM d -APV. Right: AMPAR current rectification index (−80 mV/+60 mV). ns, P = 0.5913. n = 18 cells per four mice. ( J and K ) AMPAR current rectification of WT versus WT, and WT versus Asic2 −/− neurons after social rankings. ns, P = 0.4467; *** P = 0.0003, n = 22 cells per four mice. ( L to N ) Time course of 50 μM NASPM effect on EPSCs in mPFC neurons of WT versus WT (M) and Asic2 −/− versus WT (N) mice groups before (L) and after (M and N) social rankings. NMDARs blocked with 100 μM d -APV. Right: NASPM-sensitive EPSC comparison. ns, P = 0.9864, P = 0.1059; **** P < 0.0001, n = 10 cells per four mice. All comparisons by two-tailed unpaired Student’s t test.

Techniques: Comparison, Two Tailed Test

( A ) Dendritic and spine morphology of mPFC neurons using Alexa 568 dye. ( B ) Sholl analysis for dendritic branches. Left: Parameters measured with Sholl shells from the cell body. Right: Nodes (blue dots) are branching points, and intersections (yellow dots) are where processes intersect Sholl shells. ( C ) Reconstructed mPFC neurons from WT winner and loser mice. ( D to G ) Sholl analysis of dendritic intersections (D), ** P = 0.0019; number of branches (E), * P = 0.0314; branch length (F), ns, P = 0.1465; and node numbers (G), ns, P = 0.0717. n = 6 to 8 slices per four mice. ( H ) Reconstructed mPFC neurons from Asic2 −/− winner and WT loser mice. ( I to L ) Sholl analysis: dendritic intersections (I) **** P < 0.0001; branches (J) ** P = 0.0042; branch length (K) * P = 0.0409; and nodes (L) * P = 0.0412. n = 6 to 8 slices per four mice. ( M ) Spine structures in mPFC neurons from WT winner and loser mice. ( N ) Spine density comparison: mature, **** P < 0.0001; immature, ** P = 0.001; total spines, ns, P = 0.7656. ( O ) Immature spine density (stubby, thin, and filopodia) in WT winner and loser mice. **** P < 0.0001; * P = 0.0253; ns, P = 0.9336. ( P ) Mature-to-immature spine density ratio in WT winner and loser mice, **** P < 0.0001. n = 27 to 37 neurons per four mice. ( Q ) Spine structures in mPFC neurons from Asic2 −/− winner and WT loser mice. ( R ) Spine density comparison: mature, **** P < 0.0001; immature, **** P < 0.0001; total spines, ** P = 0.0026. ( S ) Immature spine density in Asic2 −/− winner and WT loser mice, **** P < 0.0001; ns, P = 0.0624, 0.6637. ( T ) Mature-to-immature spine density ratio in Asic2 −/− winner and WT loser mice, **** P < 0.0001. n = 27 neurons per four mice. All comparisons by two-tailed unpaired Student’s t test.

Journal: Science Advances

Article Title: Mice lacking acid-sensing ion channel 2 in the medial prefrontal cortex exhibit social dominance

doi: 10.1126/sciadv.adn7573

Figure Lengend Snippet: ( A ) Dendritic and spine morphology of mPFC neurons using Alexa 568 dye. ( B ) Sholl analysis for dendritic branches. Left: Parameters measured with Sholl shells from the cell body. Right: Nodes (blue dots) are branching points, and intersections (yellow dots) are where processes intersect Sholl shells. ( C ) Reconstructed mPFC neurons from WT winner and loser mice. ( D to G ) Sholl analysis of dendritic intersections (D), ** P = 0.0019; number of branches (E), * P = 0.0314; branch length (F), ns, P = 0.1465; and node numbers (G), ns, P = 0.0717. n = 6 to 8 slices per four mice. ( H ) Reconstructed mPFC neurons from Asic2 −/− winner and WT loser mice. ( I to L ) Sholl analysis: dendritic intersections (I) **** P < 0.0001; branches (J) ** P = 0.0042; branch length (K) * P = 0.0409; and nodes (L) * P = 0.0412. n = 6 to 8 slices per four mice. ( M ) Spine structures in mPFC neurons from WT winner and loser mice. ( N ) Spine density comparison: mature, **** P < 0.0001; immature, ** P = 0.001; total spines, ns, P = 0.7656. ( O ) Immature spine density (stubby, thin, and filopodia) in WT winner and loser mice. **** P < 0.0001; * P = 0.0253; ns, P = 0.9336. ( P ) Mature-to-immature spine density ratio in WT winner and loser mice, **** P < 0.0001. n = 27 to 37 neurons per four mice. ( Q ) Spine structures in mPFC neurons from Asic2 −/− winner and WT loser mice. ( R ) Spine density comparison: mature, **** P < 0.0001; immature, **** P < 0.0001; total spines, ** P = 0.0026. ( S ) Immature spine density in Asic2 −/− winner and WT loser mice, **** P < 0.0001; ns, P = 0.0624, 0.6637. ( T ) Mature-to-immature spine density ratio in Asic2 −/− winner and WT loser mice, **** P < 0.0001. n = 27 neurons per four mice. All comparisons by two-tailed unpaired Student’s t test.

Techniques: Comparison, Two Tailed Test

( A ) Heatmap of substrate phosphorylation levels for STK in Asic2 −/− brain samples versus WT controls. ( B ) Global phosphorylation level differences between Asic2 −/− and WT brain samples. *** P = 0.0001 by paired two-tailed Student’s t test. n = 86 peptides. ( C ) Reverse KRSA plots mapping peptides to upstream kinases in Asic2 −/− versus WT brains. ( D ) Quantification of PKA and AKT ( Z > 2) using histogram peacock plots. ( E ) Waterfall plot of differentially identified kinases in Asic2 −/− brain samples versus WT controls. Red dots indicate increased or decreased representation for each kinase. ( F ) piNET analysis identified downstream proteins activated by STK pathways, highlighting PKA (PRKACA) and AKT1 pathways. Selected kinases (red nodes) and downstream regulators (blue nodes) are connected by green edges. ( G ) Asic2 −/− molecular interaction network. Functionally enriched protein-protein interaction network of upstream kinase “hits” (red) and interpolated hidden nodes (yellow) identified by Kinograte R software. n = 5 mice per group. ( H ) mRNA expression of PKA (PRKACA) via RT-qPCR in Asic2 −/− versus WT mice mPFC. * P = 0.0217 by unpaired two-tailed Student’s t test. n = 14 mice per group. ( I ) Basal cAMP level measured by ELISA in Asic2 −/− versus WT mice mPFC. ** P = 0.0039 by unpaired two-tailed Student’s t test. n = 6 to 9 mice per group. ( J ) mRNA expression of BDNF via RT-qPCR in Asic2 −/− versus WT mice mPFC. ** P = 0.0062 by unpaired two-tailed Student’s t test. n = 12 mice per group.

Journal: Science Advances

Article Title: Mice lacking acid-sensing ion channel 2 in the medial prefrontal cortex exhibit social dominance

doi: 10.1126/sciadv.adn7573

Figure Lengend Snippet: ( A ) Heatmap of substrate phosphorylation levels for STK in Asic2 −/− brain samples versus WT controls. ( B ) Global phosphorylation level differences between Asic2 −/− and WT brain samples. *** P = 0.0001 by paired two-tailed Student’s t test. n = 86 peptides. ( C ) Reverse KRSA plots mapping peptides to upstream kinases in Asic2 −/− versus WT brains. ( D ) Quantification of PKA and AKT ( Z > 2) using histogram peacock plots. ( E ) Waterfall plot of differentially identified kinases in Asic2 −/− brain samples versus WT controls. Red dots indicate increased or decreased representation for each kinase. ( F ) piNET analysis identified downstream proteins activated by STK pathways, highlighting PKA (PRKACA) and AKT1 pathways. Selected kinases (red nodes) and downstream regulators (blue nodes) are connected by green edges. ( G ) Asic2 −/− molecular interaction network. Functionally enriched protein-protein interaction network of upstream kinase “hits” (red) and interpolated hidden nodes (yellow) identified by Kinograte R software. n = 5 mice per group. ( H ) mRNA expression of PKA (PRKACA) via RT-qPCR in Asic2 −/− versus WT mice mPFC. * P = 0.0217 by unpaired two-tailed Student’s t test. n = 14 mice per group. ( I ) Basal cAMP level measured by ELISA in Asic2 −/− versus WT mice mPFC. ** P = 0.0039 by unpaired two-tailed Student’s t test. n = 6 to 9 mice per group. ( J ) mRNA expression of BDNF via RT-qPCR in Asic2 −/− versus WT mice mPFC. ** P = 0.0062 by unpaired two-tailed Student’s t test. n = 12 mice per group.

Techniques: Two Tailed Test, Software, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

( A ) Schematic showing the AAV 2 -hSyn-Cre injection into the mPFC of Asic2 f/f mice. ( B ) Representative immunofluorescence image depicting the AAV 2 -hSyn-Cre injection site. ( C ) Representative immunofluorescence image demonstrating colocalization of ASIC2 (red) and AAV 2 -hSyn-Cre/AAV 2 -hSyn-GFP (green). Please note the presence of colocalization indicated by arrows in the AAV 2 -hSyn-GFP control group (upper), whereas such colocalization is absent in the AAV 2 -hSyn-Cre group (lower). ( D ) Western blot results show ASIC2 protein levels in the AAV 2 -hSyn-Cre and AAV 2 -hSyn-GFP groups. * P = 0.0472. n = 6 mice. ( E ) Schematic showing procedures of the tube test, AAV-hSyn-Cre injection into the mPFC of Asic2 f/f mice, and the EPSC recordings. ( F ) Summarized results of ranking in the tube test before and after virus injections. ( G ) Average percentage of winning times in the tube test before and after AAV 2 -hsyn-Cre and AAV 2 -hSyn-GFP injections. *** P = 0.0007, n = 9 cages. ( H ) Representative mEPSC traces of Asic2 f/f mPFC neurons in AAV 2 -hsyn-Cre and AAV 2 -hSyn-GFP groups. ( I and J ) Cumulative distributions of mEPSC amplitudes.* P = 0.0088. ( K and L ) Cumulative distributions of mEPSC interevent intervals, * P = 0.0172. n = 10 cells from four mice per group. ( M ) Representative mIPSC traces of Asic2 f/f mPFC neurons in AAV 2 -hsyn-Cre and AAV 2 -hSyn-GFP groups. ( N and O ) Cumulative distributions of mIPSC amplitudes, ns, P = 0.8873. ( P and Q ) Cumulative distributions of mEPSC interevent intervals, * P = 0.0115. n = 9 cells from four mice per group. All comparisons by two-tailed unpaired Student’s t test.

Journal: Science Advances

Article Title: Mice lacking acid-sensing ion channel 2 in the medial prefrontal cortex exhibit social dominance

doi: 10.1126/sciadv.adn7573

Figure Lengend Snippet: ( A ) Schematic showing the AAV 2 -hSyn-Cre injection into the mPFC of Asic2 f/f mice. ( B ) Representative immunofluorescence image depicting the AAV 2 -hSyn-Cre injection site. ( C ) Representative immunofluorescence image demonstrating colocalization of ASIC2 (red) and AAV 2 -hSyn-Cre/AAV 2 -hSyn-GFP (green). Please note the presence of colocalization indicated by arrows in the AAV 2 -hSyn-GFP control group (upper), whereas such colocalization is absent in the AAV 2 -hSyn-Cre group (lower). ( D ) Western blot results show ASIC2 protein levels in the AAV 2 -hSyn-Cre and AAV 2 -hSyn-GFP groups. * P = 0.0472. n = 6 mice. ( E ) Schematic showing procedures of the tube test, AAV-hSyn-Cre injection into the mPFC of Asic2 f/f mice, and the EPSC recordings. ( F ) Summarized results of ranking in the tube test before and after virus injections. ( G ) Average percentage of winning times in the tube test before and after AAV 2 -hsyn-Cre and AAV 2 -hSyn-GFP injections. *** P = 0.0007, n = 9 cages. ( H ) Representative mEPSC traces of Asic2 f/f mPFC neurons in AAV 2 -hsyn-Cre and AAV 2 -hSyn-GFP groups. ( I and J ) Cumulative distributions of mEPSC amplitudes.* P = 0.0088. ( K and L ) Cumulative distributions of mEPSC interevent intervals, * P = 0.0172. n = 10 cells from four mice per group. ( M ) Representative mIPSC traces of Asic2 f/f mPFC neurons in AAV 2 -hsyn-Cre and AAV 2 -hSyn-GFP groups. ( N and O ) Cumulative distributions of mIPSC amplitudes, ns, P = 0.8873. ( P and Q ) Cumulative distributions of mEPSC interevent intervals, * P = 0.0115. n = 9 cells from four mice per group. All comparisons by two-tailed unpaired Student’s t test.

Techniques: Injection, Immunofluorescence, Control, Western Blot, Virus, Two Tailed Test

( A ) Generation of ASIC2-cKO mice in excitatory neurons (ASIC2-Vglut1-cKO) by crossbreeding Asic2 f/f mice with Vglut1-ires2-Cre mice. ( B ) Immunofluorescence images showing the absence of ASIC2 expression (green) in mPFC excitatory neurons labeled with N -methyl d -aspartate receptor subtype 2B (NR2B) (red). Inset highlights the lack of ASIC2 and NR2B colocalization. ( C ) One ASIC2-Vglut1-cKO and three WT mice cohoused for 2 weeks, followed by the tube test tournament to establish social rankings. Data from six cages per group summarized. ( D ) Comparison of time to stable rankings between ASIC2-Vglut1-cKO and WT subordinate mice. *** P = 0.0002, n = 6 cages. ( E ) Schematic of AAV 2 -CaMKIIα-Cre injection into Asic2 f/f mouse mPFC neurons. ( F ) Representative image of AAV 2 -CaMKIIα-Cre injection site. ( G ) Immunofluorescence image demonstrating ASIC2 (green) and AAV 2 -CaMKIIα-Cre/mCherry (red) colocalization. Arrows indicate colocalization in AAV 2 -CaMKIIα-mCherry, but not AAV 2 -CaMKIIα-Cre group. ( H ) Whole-cell patch-clamp recording of acid-induced ASIC-like currents in AAV 2 -CaMKIIα-Cre (mCherry positive) mPFC neurons. ( I ) Representative traces of acid-induced currents in AAV 2 -CaMKIIα-Cre (red) and AAV 2 -CaMKIIα-mCherry (black) neurons. ( J ) Comparison of desensitization decay times of acid-induced currents in neurons from (I). Effective ASIC2 deletion in AAV 2 -CaMKIIα-Cre neurons shows prolonged desensitization times. ** P = 0.0082. n = 15 to 17 cells from four mice per group. ( K ) Schematic of tube test procedures following AAV 2 -CaMKIIα-Cre injection into Asic2 f/f mouse mPFC. ( L ) Summary of tube test rankings before and after AAV 2 -CaMKIIα-Cre and AAV 2 -CaMKIIα-mCherry injections. ( M ) Average percentage of winning times in the tube test before and after AAV 2 -CaMKIIα-Cre and AAV 2 -CaMKIIα-mCherry injections. *** P = 0.0007, n = 6 cages. All comparisons by two-tailed unpaired Student’s t test.

Journal: Science Advances

Article Title: Mice lacking acid-sensing ion channel 2 in the medial prefrontal cortex exhibit social dominance

doi: 10.1126/sciadv.adn7573

Figure Lengend Snippet: ( A ) Generation of ASIC2-cKO mice in excitatory neurons (ASIC2-Vglut1-cKO) by crossbreeding Asic2 f/f mice with Vglut1-ires2-Cre mice. ( B ) Immunofluorescence images showing the absence of ASIC2 expression (green) in mPFC excitatory neurons labeled with N -methyl d -aspartate receptor subtype 2B (NR2B) (red). Inset highlights the lack of ASIC2 and NR2B colocalization. ( C ) One ASIC2-Vglut1-cKO and three WT mice cohoused for 2 weeks, followed by the tube test tournament to establish social rankings. Data from six cages per group summarized. ( D ) Comparison of time to stable rankings between ASIC2-Vglut1-cKO and WT subordinate mice. *** P = 0.0002, n = 6 cages. ( E ) Schematic of AAV 2 -CaMKIIα-Cre injection into Asic2 f/f mouse mPFC neurons. ( F ) Representative image of AAV 2 -CaMKIIα-Cre injection site. ( G ) Immunofluorescence image demonstrating ASIC2 (green) and AAV 2 -CaMKIIα-Cre/mCherry (red) colocalization. Arrows indicate colocalization in AAV 2 -CaMKIIα-mCherry, but not AAV 2 -CaMKIIα-Cre group. ( H ) Whole-cell patch-clamp recording of acid-induced ASIC-like currents in AAV 2 -CaMKIIα-Cre (mCherry positive) mPFC neurons. ( I ) Representative traces of acid-induced currents in AAV 2 -CaMKIIα-Cre (red) and AAV 2 -CaMKIIα-mCherry (black) neurons. ( J ) Comparison of desensitization decay times of acid-induced currents in neurons from (I). Effective ASIC2 deletion in AAV 2 -CaMKIIα-Cre neurons shows prolonged desensitization times. ** P = 0.0082. n = 15 to 17 cells from four mice per group. ( K ) Schematic of tube test procedures following AAV 2 -CaMKIIα-Cre injection into Asic2 f/f mouse mPFC. ( L ) Summary of tube test rankings before and after AAV 2 -CaMKIIα-Cre and AAV 2 -CaMKIIα-mCherry injections. ( M ) Average percentage of winning times in the tube test before and after AAV 2 -CaMKIIα-Cre and AAV 2 -CaMKIIα-mCherry injections. *** P = 0.0007, n = 6 cages. All comparisons by two-tailed unpaired Student’s t test.

Techniques: Immunofluorescence, Expressing, Labeling, Comparison, Injection, Patch Clamp, Two Tailed Test

Review

Structured Review

Images

Similar Products

Image Search Results